Human RET

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Recombinant protein corresponding to aa29-635 from human Ret/Fc Chimera expressed in Sf21 cells (P07949).

Recognizes human Ret. Species cross-reactivity: Mouse.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry (50 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Flow Cytometry, Western Blot and Direct ELISA. Flow Cytometry: 50 ug/ml; For intracellular staining to detect human Ret, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Western Blot: 1-2 ug/ml; Detection limit for rhRet is ~50 ng/lane under non-reducing and reducing conditions. Use chemiluminescence to increase sensitivity. Direct ELISA: 0.5-1.0 ug/ml; The detection limit for rhRet and rmRet is ~6 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute with 1mL sterile PBS.

Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07949 NCBI: NM_020630 NP_065681.1