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Catalog Number Size Price
LS-C38782-100 100 µg $806 
Antibody

Polyclonal Sheep anti‑Human REN / Renin 1 Antibody (aa24‑406, IHC, WB) LS‑C38782

Polyclonal Sheep anti‑Human REN / Renin 1 Antibody (aa24‑406, IHC, WB) LS‑C38782

Antibody:
REN / Renin 1 Sheep anti-Human Polyclonal (aa24-406) Antibody
Application:
IHC, WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$806
LS-C38782-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
REN / Renin 1 Sheep anti-Human Polyclonal (aa24-406) Antibody
Application:
IHC, WB, IP, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Renin 1 antibody LS-C38782 is an unconjugated sheep polyclonal antibody to human Renin 1 (REN) (aa24-406). Validated for ELISA, IHC, IP, Neut and WB.
Target
Human REN / Renin 1
Synonyms
REN | Angiotensin-forming enzyme | Angiotensinogenase | HNFJ2 | RENAA | Renin precursor, renal | Renin
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
NS0-derived rhRenin (aa24-406).
Epitope
aa24-406
Specificity
Recognizes both the pro and mature forms of human Renin. In direct ELISAs and Western Blots, this antibody shows ~20% cross-reactivity with rmRenin-1. Species sequence homology: mouse 70%, canine 72%.
Applications
  • IHC (3 - 10 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation and Neutralization. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 3-10 ug/ml. Immunoprecipitation: Used to immunoprecipitate rhRenin from conditioned media of transfected NS0 cells. Neutralization: Used to inhibit/neutralize the enzymatic activity of rhRenin. The antibody was preincubated with the activated enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by the antibody at approximately 40 ug/ml (IC50) under conditions in which the enzyme was present at 2 ug/ml and the substrate concentration was 5uM. Considering the molecular masses of the enzyme (44kD) and the antibody (150 kD), IC50 was achieved at approximately 6:1 molar ratio of the antibody to the enzyme. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Renin. The detection limit for rhRenin is approximately 3 ng/well.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About REN / Renin 1
P00797 NM_000537 NP_000528.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/24/2021