NFKB p65 antibody LS-C18904 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA). Validated for ELISA, IP and WB.
Human RELA / NFKB p65
(tested or 100% immunogen sequence identity)
Delipidated and defibrinated
- Western blot (1:1000)
NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF?B cRel is suitable for the detection by immunoblot of human NF?B cRel. No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NF?B complexes using 0.5 to 1.0 ?l per assay. Optimal titers for other applications should be determined by the researcher.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only.