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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RELA / NFKB p65 Antibody (C-Terminus) LS-C18904

NFKB p65 antibody LS-C18904 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA). Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C18904-100
100 µl (80 mg/ml)
$425
Description
NFKB p65 antibody LS-C18904 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA). Validated for ELISA, IP and WB.
Target
Human RELA / NFKB p65
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA
Epitope
C-Terminus
Specificity
NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Usage
Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF?B cRel is suitable for the detection by immunoblot of human NF?B cRel. No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NF?B complexes using 0.5 to 1.0 ?l per assay. Optimal titers for other applications should be determined by the researcher.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

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Images

Western blot

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.

Western blot

Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.
Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.

Western blot

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.

Western blot

Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.
Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.

Western blot

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.

Western blot

Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.
Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.

Western blot

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2000 for 1 h. Washes with TBS preceded color development.

Western blot

Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.
Western Blot of HeLa cell extract using anti-NFKB cRel at a dilution of 1:1000.

Requested From: United States
Date Requested: 4/23/2019
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