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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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REG3A Antibody (aa27‑175, clone 512124) LS‑C150049

Catalog Number Size Price
LS-C150049-25 25 µg $225 
LS-C150049-100 100 µg $405 
Reg3A in HepG2 Human Cell Line. Reg3A was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Reg3A Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Detection of Human Reg3A by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human Reg3A Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Reg3A at approximately 17 kD (as indicated). This experiment was conducted under reducing conditions.
Reg3A in HepG2 Human Cell Line. Reg3A was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Reg3A Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Detection of Human Reg3A by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 ug/ml of Mouse Anti-Human Reg3A Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Reg3A at approximately 17 kD (as indicated). This experiment was conducted under reducing conditions.
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REG3A Antibody (aa27‑175, clone 512124) LS‑C150049

Antibody:
Mouse Monoclonal to Human REG3A
Application:
ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$225 
LS-C150049-25
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human REG3A
Application:
ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
REG3A antibody LS-C150049 is an unconjugated mouse monoclonal antibody to human REG3A. Validated for ICC and WB.
Target
Human REG3A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
512124
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • ICC (8 - 25 µg/ml)
  • Western blot (2 µg/ml)
Immunogen
E. coli-derived recombinant human Reg3A Glu27-Asp175 Accession # Q06141.
Epitope
aa27-175
Specificity
Detects human Reg3A in direct ELISAs and Western blots.
Presentation
Lyophilized from Sterile Solution PBS, Trehalose
Reconstitution
Reconstitute in sterile PBS to 0.5 mg/ml.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About REG3A
Q06141 NM_002580 NP_002571.1

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Featured Products

Product
Species: Mouse, Rat
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Anti-REG3A antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
REG3A antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey
Applications: Western blot
Reactivity: Human
Range: 0.78-50 ng/ml
Reactivity: Mouse
Range: 15.63-1000 pg/ml
Reactivity: Monkey
Range: 15.625-1000 pg/ml

Requested From: United States
Date Requested: 8/23/2019
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