RBP4 | PRBP | Plasma retinol-binding protein | RBP | Retinol-binding protein 4
(tested or 100% immunogen sequence identity)
Protein G purified
Recognizes human RBP4. Species cross-reactivity: Recombinant mouse RBP4 25%.
- Western blot (1 - 2 µg/ml)
- Flow Cytometry
- ELISA (0.5 - 1 µg/ml)
Suitable for use in Flow Cytometry, ELISA and Western Blot. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhRBP4 is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity 5-50 fold. Flow Cytometry (HepG2 cells): 25 ug/ml For intracellular staining to detect RBP4, cells must first be fixed and permeabilized using 4% paraformaldehyde and ice-cold methanol. Add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. ELISA: 0.5-1 ug/ml. The detection limit for rhRBP4 is ~5 ng/well.
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitute in sterile PBS.
Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.