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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RBM12 Antibody (aa107‑156) LS‑C110545
RBM12 antibody LS-C110545 is an unconjugated rabbit polyclonal antibody to RBM12 from human, mouse, bat and other species. Validated for WB.
Catalog
Size
Price
LS-C110545-50
50 µg
$365

Popular RBM12 Products

Detection of Human RBM12 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RBM12 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human RBM12 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RBM12 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
RBM12 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human tonsil tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human RBM12 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RBM12 used at a dilution of 1:250.
Species: Human, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence

Product Description

RBM12 antibody LS-C110545 is an unconjugated rabbit polyclonal antibody to RBM12 from human, mouse, bat and other species. Validated for WB.

Specifications

Target
Human RBM12
Synonyms
RBM12, HRIHFB2091, KIAA0765, RNA-binding protein 12, RNA-binding motif protein 12, SWAN, RNA binding motif protein 12
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RBM12 antibody was raised against synthetic peptide located between aa107-156 of human RBM12 (Q9NTZ6, NP_006038). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Elephant, Bat (100%); Rat, Shrew, Panda, Dog, Bovine, Rabbit, Horse, Opossum, Armadillo, Lizard (92%); Guinea pig (85%); Turkey, Zebra finch, Chicken (84%).
Blocking Peptide
LS-E14860 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110545. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa107-156
Specificity
Human RBM12
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About RBM12
RBM12 encodes a protein that contains several RNA-binding motifs, potential transmembrane domains, and proline-rich regions. This gene and the gene for copine I overlap at map location 20q11.21. Alternative splicing in the 5' UTR results in four transcript variants. All variants encode the same protein. Q9NTZ6 NM_006047 CAB87611.1


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Images

Western blot

RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
RBM12 antibody Western blot of HT1080 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/13/2018

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