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Rat Anti-Mouse IgG2a Secondary Antibody (clone LO-MG2a-7, FITC) LS-C58450

Catalog Size Price
LS-C58450-500 500 µg Unavailable

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100% Guaranteed
Rat Monoclonal [clone LO-MG2a-7] (IgG1) to Mouse IgG2a
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FITC Conjugated

Details

Mouse IgG2a
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [LO-MG2a-7]
Fluorescein
Affinity purified
Unmodified

Applications

Flow Cytometry (5 µg/ml)

Specificity and Use

Specific for the gamma 2a heavy chain of mouse Ig. Note: Recognizes an allotypic determinant upon mouse IgG2a. It does recognize the IgH Ia allotype (as expressed in Balb/c mice), but not the IgH Ib allotype (as expressed in C57/BL mice).
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.09% sodium azide, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Mouse IgG2a

The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope — the antigen-binding site — at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens. In an experimental setting, Fc and Fab fragments can be generated in the laboratory.

Requested From: 
Date Requested: 12/11/2017

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