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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAPGEF5 / GFR Antibody (Internal) LS‑C353196
GFR antibody LS-C353196 is an unconjugated rabbit polyclonal antibody to GFR (RAPGEF5) from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C353196-100
100 µl (1 mg/ml)
$245

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Product Description

GFR antibody LS-C353196 is an unconjugated rabbit polyclonal antibody to GFR (RAPGEF5) from human, mouse and rat. Validated for WB.

Specifications

Target
Human RAPGEF5 / GFR
Synonyms
RAPGEF5, GFR, KIAA0277, M-Ras-regulated Rap GEF, M-Ras-regulated GEF, MRGEF, Related to Epac, REPAC, MR-GEF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
Immunogen
RAPGEF5 / GFR antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the central region of human GFR.
Blocking Peptide
LS-E42946 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C353196. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of GFR protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About RAPGEF5 / GFR
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]). Q92565 D87467 BAA13406.2


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Images

Western blot

Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

Western blot

Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

Western blot

Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

Western blot

Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Western blot analysis of GFR expression in K562 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.

Requested From: United States
Date Requested: 11/16/2018

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