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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C162428-400 400 µl $325 
RAN Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
RAN Antibody - Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
RAN Antibody - Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
RAN Antibody - Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
RAN Antibody - RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAN Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
RAN Antibody - Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
RAN Antibody - Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
RAN Antibody - Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
RAN Antibody - RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human RAN Antibody (aa12‑39, IHC, WB) LS‑C162428

Polyclonal Rabbit anti‑Human RAN Antibody (aa12‑39, IHC, WB) LS‑C162428

Antibody:
Rabbit Polyclonal to Human RAN
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C162428-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human RAN
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RAN antibody LS-C162428 is an unconjugated rabbit polyclonal antibody to RAN (aa12-39) from human. It is reactive with human and mouse. Validated for Flow, IHC and WB.
Target
Human RAN
Synonyms
RAN | Gsp1 | Guanosine triphosphatase Ran | RanGTPase | Member RAS oncogene family | OK/SW-cl.81 | Ras-related nuclear protein | ARA24 | TC4 | GTPase Ran | Ras-like protein TC4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Chicken, Xenopus, Zebrafish, Yeast, C. elegans, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8389 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C162428. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa12-39
Specificity
This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human RAN.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAN
P62826 NM_006325 NP_006316.1

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Requested From: United States
Date Requested: 5/28/2020