LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

RAMA1 / SKA3 Antibody IHC-plus™ LS-B5039

Best Seller
Note: This antibody replaces LS-C108594
SKA3 antibody LS-B5039 is an unconjugated rabbit polyclonal antibody to SKA3 (RAMA1) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5039-0.05
0.05 mg (1 mg/ml)
$425
Description
SKA3 antibody LS-B5039 is an unconjugated rabbit polyclonal antibody to SKA3 (RAMA1) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RAMA1 / SKA3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAMA1 / SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3.
Blocking Peptide
LS-E30780 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B5039. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About RAMA1 / SKA3
Q8IX90 NM_145061 NP_659498.4

Popular RAMA1 / SKA3 Products

Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
C13orf3 Antibody IHC of formalin-fixed and paraffin-embedded testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA

Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunohistochemistry

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.

Requested From: United States
Date Requested: 5/19/2019
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy