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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAMA1 / SKA3 Antibody IHC-plus™ LS-B5039

Note: This antibody replaces LS-C108594
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of SKA3 in human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/ml.
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RAMA1 / SKA3 Antibody IHC-plus™ LS-B5039

Note: This antibody replaces LS-C108594
Antibody:
Rabbit Polyclonal to Human RAMA1 / SKA3
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5039-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RAMA1 / SKA3
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SKA3 antibody LS-B5039 is an unconjugated rabbit polyclonal antibody to SKA3 (RAMA1) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human RAMA1 / SKA3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAMA1 / SKA3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3.
Blocking Peptide
LS-E30780 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B5039. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
SKA3 antibody can be used for detection of SKA3 by Western blot at 0.5 - 1 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAMA1 / SKA3
Q8IX90 NM_145061 NP_659498.4

Validation

Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Featured Products

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Cynomolgus
Range: 0.7-10 ng/ml
Reactivity: Mouse
Range: 15.63-1000 pg/ml

Requested From: United States
Date Requested: 7/19/2019
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