RAF antibody LS-C9899 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for ICC and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
RAF1 / RAF antibody was raised against produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains tyrosines 340 and 341. The sequence is conserved in mouse and rat.
Reactivity: Human c-Raf. Mouse and rat c-Raf (100% homologous) have not been tested, but are expected to react.
Suitable for use in Western blotting applications. Previous lots of this antibody have been used in immunocytochemistry. The current lot has not yet been validated for this application. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control: HEK293 overexpressing c-Raf, in vitro-phosphorylated by active Src.
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.