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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAF1 / RAF Antibody (phospho-Tyr340/Tyr341) LS-C9899

RAF antibody LS-C9899 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for ICC and WB.
Catalog
Size
Price
LS-C9899-10
10 blot
$635
Description
RAF antibody LS-C9899 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for ICC and WB.
Target
Human RAF1 / RAF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
Immunogen
RAF1 / RAF antibody was raised against produced against a chemically synthesized phosphopeptide derived from a region of human c-Raf that contains tyrosines 340 and 341. The sequence is conserved in mouse and rat.
Epitope
pTyr340/Tyr341
Specificity
Reactivity: Human c-Raf. Mouse and rat c-Raf (100% homologous) have not been tested, but are expected to react.
Usage
Suitable for use in Western blotting applications. Previous lots of this antibody have been used in immunocytochemistry. The current lot has not yet been validated for this application. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control: HEK293 overexpressing c-Raf, in vitro-phosphorylated by active Src.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

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Requested From: United States
Date Requested: 4/21/2019
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