RAF antibody LS-C9889 is an unconjugated rat monoclonal antibody to RAF (RAF1). Validated for WB.
Also available conjugated with AP
RAF1 / RAF antibody was raised against synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde.
Recognizes activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339. May be used for Western blotting detection of activated Raf-1 as described by Mason et al.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.