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RAF1 / RAF Antibody (C‑Terminus) LS‑C9881

Antibody

RAF1 / RAF Antibody (C‑Terminus) LS‑C9881

Antibody:
Rabbit Polyclonal to Human RAF1 / RAF
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$560 
LS-C9881-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human RAF1 / RAF
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
RAF antibody LS-C9881 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for ELISA, IP and WB.
Target
Human RAF1 / RAF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
RAF1 / RAF antibody was raised against a 12 amino acid synthetic peptide derived from the extreme carboxy-terminus of the human Raf-1 protein.
Epitope
C-Terminus
Specificity
Can be used to specifically immunoprecipitate the ~74kD Raf-1 protein from native cell lysates. Best suited for immunoprecipitation (IP) or IP/Western applications. When used for straight Western Blot, cross-reactivity with a doublet at ~86kD is sometimes observed. Species cross-reactivity: human, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Suggested positive control lysates include HeLa (human) and NIH 3T3 (mouse) cell lines. ELISA: 0.1-1.0 ug/ml. Western Blot (following IP): 2 ug/ml. Immunoprecipitation: 10 ug/IP reaction.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

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Requested From: United States
Date Requested: 7/20/2019
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