RAF antibody LS-C9881 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) from human, mouse and rat. Validated for ELISA, IP and WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (2 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
RAF1 / RAF antibody was raised against a 12 amino acid synthetic peptide derived from the extreme carboxy-terminus of the human Raf-1 protein.
Can be used to specifically immunoprecipitate the ~74kD Raf-1 protein from native cell lysates. Best suited for immunoprecipitation (IP) or IP/Western applications. When used for straight Western Blot, cross-reactivity with a doublet at ~86kD is sometimes observed. Species cross-reactivity: human, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Suggested positive control lysates include HeLa (human) and NIH 3T3 (mouse) cell lines. ELISA: 0.1-1.0 ug/ml. Western Blot (following IP): 2 ug/ml. Immunoprecipitation: 10 ug/IP reaction.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.