Yeast RAD9A / RAD9
RAD9A | HRAD9 | RAD9 | RAD9 homolog | RAD9 (S. pombe) homolog | RAD9 homolog A (S. pombe)
(tested or 100% immunogen sequence identity)
Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the ortholog of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. Is pan reactive with both the phosphorylated and non-phosphorylated forms of this protein.