RAD50 antibody LS-C17557 is an unconjugated mouse monoclonal antibody to human RAD50. Validated for IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
RAD50 antibody was raised against gST fusion protein corresponding to residues 1-425 of human RAD50. The immunogen shares 389/425 identity with mouse and 127/425 identity with yeast.
Recognizes human Rad50.
Western Blot Analysis: 0.5-2 ug/ml detects RAD50 in a HeLa nuclear lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Rad50 (0.5 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear lysate. Use 20 ug per lane for minigels. Immunoprecipitation: Reported to immunoprecipitate.
10 mM PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.