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Antibodies > Mouse IgG > LS-C60800
 
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Catalog Number Size Price
LS-C60800-2 2 mg (2 mg/ml) $333 
Mouse IgG Antibody - ELISA results of purified Rabbit Anti-Mouse IgG Biotin Conjugated Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate 1:10,000, and TMB-8000 substrate.

Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60800

Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Biotin, IHC, WB) LS‑C60800

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Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$333
LS-C60800-2
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Biotin, Unmodified

Specifications

Description
Mouse IgG antibody LS-C60800 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Mouse IgG
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:300000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Mouse IgG Antibody - Mouse IgG (H&L) Antibody Peroxidase Conjugated - Western Blot. Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated Lane 1: Mouse IgG Load: 50 ng per lane Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 and 28 kD for Mouse IgG. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Donkey Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60841
Species: Mouse
Applications: IHC, Western blot, ELISA
Mouse IgG Antibody - ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Gt-anti-Donkey HRP conjugate and TMB-1000 substrate.
Goat Anti‑Mouse IgG Secondary Antibody LS‑C60700
Species: Mouse
Applications: IHC, Western blot, ELISA
Mouse IgG Antibody - Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60710
Species: Mouse
Applications: IHC, Western blot, ELISA
Product
Goat Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C55886
Species: Mouse
Applications: ELISA
Product
Rat Anti‑Mouse IgG Secondary Antibody (clone LO‑MG‑7, Concentrated) LS‑C202226
Species: Mouse
Applications: IHC, IHC - Frozen, ELISA
Mouse IgG Antibody - Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60680
Species: Mouse
Applications: IHC, Western blot, ELISA
See all Mouse IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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