Sheep IgM

Rabbit

Sheep (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Delipidation, salt fractionation and ion exchange chromatography followed by cross adsorptions and dialysis.

Unmodified

Sheep IgM mu heavy chain

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgM and Sheep Serum. No reaction was observed against Sheep IgG.

• IHC (1:500 - 1:2500)
• Western blot (1:1000 - 1:10000)
• ELISA (1:10000 - 1:50000)

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. This product has been assayed against 1.0 ug of Sheep IgM in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 2 mL of deionized water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.