Mouse IgM

Rabbit

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Alkaline Phosphatase

Immunoaffinity purified

Unmodified

Mouse IgM heavy chain.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against Mouse IgG F(c).

• IHC
• Western blot
• ELISA

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilution (s): This product has been assayed against 5.0 ug of Mouse IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Tris chloride, 0.001 M magnesiuim chloride, 0.001 M zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.