(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:2000 - 1:4000)
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Mouse IgG3 antibody was raised against mouse IgG3 heavy chain.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes.
Suitable for Western Blot (Western or Dot Blot), ELISA and immunohistochemistry. ELISA: 1:2000-1:4000. Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide, 50% glycerol.
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.