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Rabbit Anti-Mouse IgG Secondary Antibody (Fab'2, FITC) LS-C59206

Catalog Size Price
LS-C59206-1000 1000 µg Unavailable

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548 Mouse IgG Antibodies

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Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Mouse IgG (H&L) Antibody Peroxidase Conjugated - Western Blot. Western Blot of Donkey anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated Lane 1: Mouse IgG Load: 50 ng per lane Secondary antibody: Mouse IgG (H&L) Antibody Peroxidase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 and 28 kD for Mouse IgG. This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse IgG
Mouse, Rat
Flow Cytometry
FITC Conjugated, Fab'2

Details

Mouse IgG
Rabbit
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Fluorescein
Affinity purified
Fab'2

Applications

Flow Cytometry (1:200 - 1:500)

Specificity and Use

Mouse IgG antibody was raised against purified mouse IgG.
Mouse IgG (all subclasses). Cross reactivity with mouse IgM and IgA is expected. Cross reactivity with rat IgG is expected. Cross reactivity with human serum proteins has been minimized by solid phase adsorption.
Flow Cytometry: Use 50 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

Packaging

PBS, pH 7.2, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Mouse IgG

Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds.

Requested From: 
Date Requested: 4/19/2018

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