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Catalog Number Size Price
LS-C745589-100 100 µg (1 mg/ml) $272 
Mouse IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Atto 647N, IF, WB) LS‑C745589

Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Atto 647N, IF, WB) LS‑C745589

Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified
Price
Catalog Number
$272
LS-C745589-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Atto 647N) Antibody
Application:
IF, WB, FLISA
Reactivity:
Mouse
Format:
Atto 647N, Unmodified

Specifications

Description
Mouse IgG antibody LS-C745589 is an Atto 647N-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 647N
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Specificity
This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021