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Antibodies > Mouse IgG > LS-C745587
 
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Catalog Number Size Price
LS-C745587-100 100 µg (1 mg/ml) $294 
Mouse IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Mouse IgG Antibody - Western Blot of ATTO 594 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 594 rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG Subclasses. Other band(s): None.
Mouse IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Mouse IgG Antibody - Western Blot of ATTO 594 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 594 rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG Subclasses. Other band(s): None.
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Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Atto 594, IF, WB) LS‑C745587

Polyclonal Rabbit Anti‑Mouse IgG Secondary Antibody (Atto 594, IF, WB) LS‑C745587

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Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Atto 594) Antibody
Application:
IF, WB, FLISA
Reactivity:
Mouse
Format:
Atto 594, Unmodified
Price
Catalog Number
$294
LS-C745587-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Mouse IgG Rabbit anti-Mouse Polyclonal (Atto 594) Antibody
Application:
IF, WB, FLISA
Reactivity:
Mouse
Format:
Atto 594, Unmodified

Specifications

Description
Mouse IgG antibody LS-C745587 is an Atto 594-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
Target
Mouse IgG
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 594
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins.
Specificity
This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Donkey Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60841
Species: Mouse
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Mouse IgG Antibody - ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Gt-anti-Donkey HRP conjugate and TMB-1000 substrate.
Goat Anti‑Mouse IgG Secondary Antibody LS‑C60700
Species: Mouse
Applications: IHC, Western blot, ELISA
Mouse IgG Antibody - Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 30 min at RT. Predicted/Observed size: 50 kDa, 25 kDa for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60710
Species: Mouse
Applications: IHC, Western blot, ELISA
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Species: Mouse
Applications: IHC, IHC - Frozen, ELISA
Mouse IgG Antibody - Western Blot of Peroxidase conjugated Goat anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Mouse IgG Secondary Antibody (HRP) LS‑C60680
Species: Mouse
Applications: IHC, Western blot, ELISA
See all Mouse IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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