Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Alkaline Phosphatase (AP)
IHC, Western blot, ELISA (1:4000 - 1:16000)
Specificity and Use
Mouse IgG antibody was raised against mouse IgG1 heavy chain.
anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50% glycerol, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.
Requested From: United States
Date Requested: 7/6/2015