(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:4000 - 1:16000)
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Mouse IgG antibody was raised against mouse IgG1 heavy chain.
anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50% glycerol, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Store at 4°C. Do not freeze.
For research use only.
This antibody carries the LSBio 100% Guarantee.