Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
IHC, Western blot, ELISA (1:4000 - 1:16000)
Specificity and Use
Mouse IgG antibody was raised against mouse IgG1 heavy chain.
anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature.
0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50% glycerol, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Store at 4°C. Do not freeze.
For research use only.
About Mouse IgG
Representing approximately 75% of serum antibodies, IgG is the most common type of antibody found in the circulation. IgG molecules are created and released by plasma B cells. IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class gamma heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The two heavy chains are linked to each other and to a light chain each by disulfide bonds.
Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.
Requested From: United States
Date Requested: 5/30/2016