Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Specificity and Use
Mouse IgG, IgM and IgA.
Antibodies were raised in rabbit to mouse IgG, IgM and IgA. Specific antibodies were absorpted by incubation with Sepharose-bound mouse immuno- globulins. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized mouse immunoglobulins a minimum of 65% protein bound. The purified polyclonal was labeled with fluorescein isothiocyanate isomer I followed by gel-filtration and ion-exchange chromatography to clear unbound conjugate. F/P ratio: 3-5 mol Fluorescein per 1 mol rabbit IgG. Low background fluorescence of normal tissue in contrast to specific staining of sections treated with monoclonal mouse anti-human collagen antibodies. No cross reactivity with albumin fractions of mouse serum. No cross reactivity with albumin fractions of animal species.
For immunohistochemical staining of tissue sections a 1:40 dilution is recommended.
50% glycerol, 0.1 M sodium chloride, 0.0 1 M sodium phosphate at, pH 7.5