Mouse IgM, Fc

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Tetramethyl Rhodamine Iso-Thiocyanate. Also available Unconjugated or conjugated with Biotin, FITC, HRP.

Purified IgG

Unmodified

Purified pools of homogenous IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Mouse IgM, Fc specific.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence
• ELISA
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:80.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.