Human IgG Fc

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Affinity chromatography

Unmodified

Human IgG F(c) fragment.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).

• IHC (1:500 - 1:2500)
• Western blot (1:1000 - 1:5000)
• ELISA (1:10000 - 1:50000)
• Dot Blot
• Electron Microscopy

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Human IgG F(c) in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. Researchers should determine optimal titers for other applications. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 1 mL of deionized water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles, store undiluted. Expiration date is 1 year from date of restoration.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.