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Rabbit Anti‑Hemagglutinin / HA Tag Secondary Antibody (HRP) LS‑C154276

Catalog Number Size Price
LS-C154276-100 100 µg (1 mg/ml) $425 
Antibody

Rabbit Anti‑Hemagglutinin / HA Tag Secondary Antibody (HRP) LS‑C154276

Antibody:
Rabbit Polyclonal to Hemagglutinin / HA Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154276-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Hemagglutinin / HA Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
HA Tag antibody LS-C154276 is an HRP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Hemagglutinin / HA Tag
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:1000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the C-terminus end to facilitate coupling.
Epitope
C-Terminus
Specificity
This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Usage
Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 8/20/2019
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