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| Catalog Number | Size | Price |
|---|---|---|
| LS-C346884-1 | 1 ml | $438 |
Polyclonal Rabbit Anti‑Goat IgM Secondary Antibody (Biotin, IHC) LS‑C346884
Polyclonal Rabbit Anti‑Goat IgM Secondary Antibody (Biotin, IHC) LS‑C346884
Antibody:
Goat IgM Rabbit anti-Goat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Goat
Format:
Biotin, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Goat IgM Rabbit anti-Goat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB, ID
Reactivity:
Goat
Format:
Biotin, Unmodified
Other formats:
Specifications
Description
Goat IgM antibody LS-C346884 is a biotin-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, ICC, ID and IHC.
Host
Rabbit
Reactivity
Goat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified normal IgM isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Goat IgM, heavy and light chains.
Applications
- IHC
- IHC - Paraffin
- ICC
- ELISA
- Dot Blot
- Immunodiffusion
- Applications tested for the base form of this product only
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgM molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in goat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Goat
Applications:
IHC, Western blot, ELISA
Species:
Goat
Applications:
IHC, IHC - Paraffin, ICC, ELISA, Dot Blot, Immunodiffusion
Species:
Goat
Applications:
IHC, IHC - Frozen, ICC, Immunofluorescence, ELISA, Immunodiffusion
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
