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Catalog Number Size Price
LS-C745569-100 100 µg (1 mg/ml) $275 
Goat IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Goat IgG Antibody - Western Blot of Atto 594 Conjugated Rabbit Anti-Goat IgG Pre-Absorbed Secondary Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 rabbit secondary antibody at 1:1,000 in MB-070 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Goat IgG. Other band(s): None.
Goat IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Goat IgG Antibody - Western Blot of Atto 594 Conjugated Rabbit Anti-Goat IgG Pre-Absorbed Secondary Antibody. Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 rabbit secondary antibody at 1:1,000 in MB-070 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Goat IgG. Other band(s): None.
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Polyclonal Rabbit Anti‑Goat IgG Secondary Antibody (Atto 594, IF, WB) LS‑C745569

Polyclonal Rabbit Anti‑Goat IgG Secondary Antibody (Atto 594, IF, WB) LS‑C745569

Antibody:
Goat IgG Rabbit anti-Goat Polyclonal (Atto 594) Antibody
Application:
IF, WB, FLISA
Reactivity:
Goat
Format:
Atto 594, Unmodified
Price
Catalog Number
$275
LS-C745569-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat IgG Rabbit anti-Goat Polyclonal (Atto 594) Antibody
Application:
IF, WB, FLISA
Reactivity:
Goat
Format:
Atto 594, Unmodified

Specifications

Description
Goat IgG antibody LS-C745569 is an Atto 594-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
Host
Rabbit
Reactivity
Goat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 594
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse or Rabbit Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 5/12/2021