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Antibodies > Goat IgG > LS-C745568
 
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Catalog Number Size Price
LS-C745568-100 100 µg (1 mg/ml) $310 
Goat IgG Antibody - Atto dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue).
Goat IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Goat IgG Antibody - Western Blot of Atto 550 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Atto 550 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.
Goat IgG Antibody - Atto dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue).
Goat IgG Antibody - ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Goat IgG Antibody - Western Blot of Atto 550 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Atto 550 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.
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Polyclonal Rabbit Anti‑Goat IgG Secondary Antibody (Atto 550, IF, WB) LS‑C745568

Polyclonal Rabbit Anti‑Goat IgG Secondary Antibody (Atto 550, IF, WB) LS‑C745568

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Antibody:
Goat IgG Rabbit anti-Goat Polyclonal (Atto 550) Antibody
Application:
IF, WB, FLISA
Reactivity:
Goat
Format:
Atto 550, Unmodified
Price
Catalog Number
$310
LS-C745568-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Goat IgG Rabbit anti-Goat Polyclonal (Atto 550) Antibody
Application:
IF, WB, FLISA
Reactivity:
Goat
Format:
Atto 550, Unmodified

Specifications

Description
Goat IgG antibody LS-C745568 is an Atto 550-conjugated rabbit polyclonal antibody. Validated for FLISA, IF and WB.
Target
Goat IgG
Host
Rabbit
Reactivity
Goat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 550
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human, Mouse or Rabbit Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

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Applications: IHC - Paraffin, IHC - Frozen, Western blot, ELISA
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Donkey Anti‑Goat IgG Secondary Antibody (Biotin) LS‑C59905
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Applications: IHC, Western blot, ELISA
Goat IgG Antibody - Western Blot - Goat IgG (H&L) Antibody Fluorescein Conjugated. Western Blot of Rabbit anti-Goat IgG (H&L) Antibody Fluorescein Conjugated Lane 1: Goat IgG Load: 50 ng per lane Secondary antibody: Goat IgG (H&L) Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kD, 55 and 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit Anti‑Goat IgG Secondary Antibody (FITC) LS‑C60335
Species: Goat
Applications: Immunofluorescence, Western blot, Flow Cytometry, Fluorophore-Linked Immunosorbent Assay
See all Goat IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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