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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154275-100 100 µg (1 mg/ml) $425 
DYKDDDDK Tag Antibody - IR700DX - Western Blot. Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. Protein was run on a 4-20% gel, transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20C, primary antibody was used at 1:2500 overnight at 4C. IRDye700DX secondary antibody was used at 1:20000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kD molecular weight position expected for alpha tubulin. This image was taken for the unconjugated form of this product. Other forms have not been tested.

DYKDDDDK Tag Antibody (C‑Terminus, HRP) LS‑C154275

DYKDDDDK Tag Antibody (C‑Terminus, HRP) LS‑C154275

Antibody:
Rabbit Polyclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154275-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C154275 is an HRP-conjugated rabbit polyclonal antibody to DYKDDDDK Tag. Validated for ELISA, IHC and WB.
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:2000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the C-terminus end to facilitate coupling. This antibody reacts with FLAG conjugated proteins.
Epitope
C-Terminus
Specificity
This affinity purified antibody is directed against DYKDDDDK and is useful in determining its presence in various assays. This polyclonal antibody detects over-expressed proteins containing DYKDDDDK at either the N or C terminus. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Usage
This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to the amino- and carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and DYKDDDDK containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. This polyclonal antibody to detect DYKDDDDK conjugated proteins binds DYKDDDDK containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/12/2019