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Rabbit Anti‑DYKDDDDK Tag Secondary Antibody (Biotin) LS‑C154292

Catalog Number Size Price
LS-C154292-100 100 µg (1 mg/ml) $425 
Antibody

Rabbit Anti‑DYKDDDDK Tag Secondary Antibody (Biotin) LS‑C154292

Antibody:
Rabbit Polyclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Biotin, Unmodified
Price
Catalog Number
$425 
LS-C154292-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to DYKDDDDK Tag
Application:
IHC, WB, ELISA
Format:
Biotin, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C154292 is a biotin-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the C-terminus end to facilitate coupling. This antibody reacts with FLAG? conjugated proteins.
Epitope
C-Terminus
Specificity
This affinity purified antibody is directed against DYKDDDDK and is useful in determining its presence in various assays. This polyclonal antibody detects over-expressed proteins containing DYKDDDDK. To date this antibody has reacted with all amino-terminal DYKDDDDK tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Usage
This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to the carboxy or amino- terminus of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and DYKDDDDK containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. This polyclonal antibody to detect DYKDDDDK conjugated proteins binds DYKDDDDK containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 1% BSA
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Featured Products

DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
Species:
Applications: IHC, Western blot, ELISA
Western blot Image
Species:
Applications: Western blot, Immunoprecipitation
Product
Species:
Applications: Western blot, ELISA

Requested From: United States
Date Requested: 8/22/2019
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