Dog IgM

Rabbit

Dog (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

Dog IgM mu heavy chain

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgM and Dog Serum. No reaction was observed against Dog IgG.

• IHC (1:500 - 1:2500)
• Western blot (1:1000 - 1:10000)
• ELISA (1:10000 - 1:50000)
• Dot Blot

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. This product has been assayed against 1.0 ug of Dog IgM in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 2 mL of deionized water.

Store lyophilized at 4°C. Once reconstituted, aliquot and store at 20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.