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Antibodies > RAB3B > LS-C392561
 
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Catalog Number Size Price
LS-C392561-200 200 µl $393 
RAB3B Antibody - Antibody staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
RAB3B Antibody - All lanes: Anti-RAB3B Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate. Lane 3: SW480 whole cell lysate. Lane 4: human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3B Antibody - Overlay histogram showing HepG2 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
RAB3B Antibody - Antibody staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
RAB3B Antibody - All lanes: Anti-RAB3B Antibody at 1:2000 dilution. Lane 1: mouse brain lysate. Lane 2: rat brain lysate. Lane 3: SW480 whole cell lysate. Lane 4: human brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RAB3B Antibody - Overlay histogram showing HepG2 cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Monoclonal Mouse anti‑Human RAB3B Antibody (clone 1543CT354.60.92, IHC, WB) LS‑C392561

Monoclonal Mouse anti‑Human RAB3B Antibody (clone 1543CT354.60.92, IHC, WB) LS‑C392561

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Antibody:
RAB3B Mouse anti-Human Monoclonal (1543CT354.60.92) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392561-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAB3B Mouse anti-Human Monoclonal (1543CT354.60.92) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RAB3B antibody LS-C392561 is an unconjugated mouse monoclonal antibody to RAB3B from human. It is reactive with human, mouse and rat. Validated for Flow, IHC, Peptide-ELISA and WB.
Target
Human RAB3B
Synonyms
RAB3B | Ras-related protein Rab-3B | Brain antigen RAB3B
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1543CT354.60.92
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAB3B
P20337 NM_002867 NP_002858.2

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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