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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C355628-200 200 µg $445 
Rab11 Antibody - Immunofluorescent analysis of Rab11B (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Rab11B rabbit polyclonal antibody at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Rab11 Antibody - WB using RAB11 Antibody
Rab11 Antibody - Immunofluorescent analysis of Rab11B (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Rab11B rabbit polyclonal antibody at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Rab11 Antibody - WB using RAB11 Antibody
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Rab11 Antibody LS‑C355628

Rab11 Antibody LS‑C355628

Antibody:
Rabbit Polyclonal to Human Rab11
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$445 
LS-C355628-200
200 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Rab11
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Rab11 antibody LS-C355628 is an unconjugated rabbit polyclonal antibody to human Rab11. Validated for IF and WB.
Target
Human Rab11
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Gibbon, Monkey, Mouse, Dog, Goat, Guinea pig, Horse, Pig, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (6.6 µg/ml)
Immunogen
Synthetic peptide corresponding to aa1-17 (MGTRDDEYDYLFKVVLIC) of human Rab11. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Dog, Cat, Goat, Elephant, Horse, Pig, Guinea pig, Chicken, Xenopus, Medaka, Pufferfish, Zebrafish (94%).
Specificity
The antibody detects Rab 11 from human and non-human primate samples. This antibody detects both A and B isoforms. It has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~22 kD protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kD.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/14/2019