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Catalog Number Size Price
LS-C815018-100 100 µl $412 
PYGO2 / Pygopus 2 Antibody - Immunofluorescence staining of PYGO2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PYGO2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.
PYGO2 / Pygopus 2 Antibody - Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa.
PYGO2 / Pygopus 2 Antibody - PYGO2 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. 1 uL anti-PYGO2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PYGO2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 54 kDa.
PYGO2 / Pygopus 2 Antibody - Immunofluorescence staining of PYGO2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PYGO2 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.
PYGO2 / Pygopus 2 Antibody - Anti-PYGO2 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa.
PYGO2 / Pygopus 2 Antibody - PYGO2 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. 1 uL anti-PYGO2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PYGO2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 54 kDa.
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Polyclonal Rabbit anti‑Human PYGO2 / Pygopus 2 Antibody (Preservative Free, IF, WB) LS‑C815018

Polyclonal Rabbit anti‑Human PYGO2 / Pygopus 2 Antibody (Preservative Free, IF, WB) LS‑C815018

Antibody:
PYGO2 / Pygopus 2 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C815018-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PYGO2 / Pygopus 2 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
Pygopus 2 antibody LS-C815018 is an unconjugated rabbit polyclonal antibody to human Pygopus 2 (PYGO2). Validated for ICC, IF, IP and WB.
Target
Human PYGO2 / Pygopus 2
Synonyms
PYGO2 | Pygopus homolog 2 (Drosophila) | Pygopus 2 | Pygopus homolog 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the center region of the Human Pygopus-2/PYGO2
Specificity
Human Pygopus-2/PYGO2
Applications
  • ICC (1:500 - 1:2500)
  • Immunofluorescence (1:500 - 1:2500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PYGO2 / Pygopus 2
Q9BRQ0 AA987476 NP_612157.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/23/2024