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PUM1

pumilio RNA-binding family member 1

PUM1 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene Name: pumilio RNA-binding family member 1
Synonyms: PUM1, KIAA0099, PUML1, Pumilio homolog 1, Pumilio homolog 1 (Drosophila), HSPUM, PUMH, PUMH1, Pumilio (Drosophila) homolog 1, Pumilio-1
Target Sequences: NM_014676 NP_055491.1 Q14671

☰ Filters
Antibodies (23)
Proteins (3)
Over-Expression Lysate (2)
Primary (23)
Recombinant (1)
PUM1 (26)
Human (23)
Mouse (7)
Rat (1)
IHC (16)
IHC-P (2)
WB (21)
ELISA (12)
ICC (1)
IF (2)
IP (3)
Peptide-ELISA (1)
rabbit (22)
goat (1)
IgG (21)
polyclonal pc (23)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (2)
Unconjugated (13)
Tyr83 (7)
aa1-30 (6)
aa1-50 (1)
aa200-250 (1)
aa225-275 (1)
No (26)
Myc-DDK (Flag) (3)
Human (2)
293T Cells (1)
HEK 293 Cells (2)
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-30) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
PUM1 Antibody - Immunofluorescence staining of PUM1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PUM1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF
Unconjugated
100 µl/$412
PUM1 Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with Phospho-PUM1-Y83.control antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Select
PUM1 Rabbit anti-Human Polyclonal (Tyr83) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
PUM1 Antibody - Immunohistochemical analysis of Pumilio 1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
PUM1 Antibody - Immunofluorescence analysis of U2OS cells using pum1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PUM1 Antibody - Immunohistochemistry of paraffin-embedded mouse brain tissue.
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Select
PUM1 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
PUM1 Antibody - Western blot analysis of PUM1 using HuvEc whole lysates.
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PUM1 Antibody - Detection of Human and Mouse Pumilio 1 by Western Blot. Samples: RIPA extract from HeLa (10 ug) and 3T3 (50 ug) cells. Antibody: Affinity purified goat anti-Pumilio 1 used at 0.03 ug/ml for HeLa extract and 0.1 ug/ml for 3T3 extract. Detection: Chemiluminescence with 1 minute exposure. (CIP: Calf Intestinal Phosphatase).
Select
PUM1 Goat anti-Human Polyclonal (aa225-275) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
Select
PUM1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
PUM1 Rabbit anti-Human Polyclonal (aa200-250) Antibody
Human
IP
Unconjugated
10 µg/$295; 100 µl/$514
Select
293T Cells
Myc-DDK (Flag)
126.3 kDa
20 µg/$150
PUM1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
126.3 kDa
100 µg/$494
Viewing 1-25 of 26 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).