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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PTPRF Antibody IHC‑plus™ LS‑B15519
Note: This antibody replaces LS-C408840
PTPRF antibody LS-B15519 is an unconjugated rabbit polyclonal antibody to PTPRF from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B15519-50
50 µl
$425
Description
PTPRF antibody LS-B15519 is an unconjugated rabbit polyclonal antibody to PTPRF from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PTPRF
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PTPRF antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human PTPRF (NP_569707.2). DSKPVFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSG
Specificity
Human PTPRF.
Usage
The predicted MW is 211kDa/212kDa, while the observed MW by Western blot was 150kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PTPRF
P10586 NM_002840 NP_002831.2

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Images

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Western blot

Western blot blot of extracts of various cells, using PTPRF antibody.
Western blot blot of extracts of various cells, using PTPRF antibody.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Western blot

Western blot blot of extracts of various cells, using PTPRF antibody.
Western blot blot of extracts of various cells, using PTPRF antibody.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Western blot

Western blot blot of extracts of various cells, using PTPRF antibody.
Western blot blot of extracts of various cells, using PTPRF antibody.

Immunohistochemistry - Paraffin

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Immunohistochemistry - Paraffin

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE), At a dilution of 1:100.

Western blot

Western blot blot of extracts of various cells, using PTPRF antibody.
Western blot blot of extracts of various cells, using PTPRF antibody.

Requested From: United States
Date Requested: 12/15/2018
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