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Catalog Number Size Price
LS-C801159-100 100 µl (1 mg/ml) $379 
LS-C801159-200 200 µl (1 mg/ml) $421 
PTPN11 / SHP-2 / NS1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PTPN11 / SHP-2 / NS1 Antibody - Staining A431 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PTPN11 / SHP-2 / NS1 Antibody - Western blot analysis of SHP-2 phosphorylation expression in A431 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PTPN11 / SHP-2 / NS1 Antibody - Western blot analysis of Phospho-SHP-2 (Tyr542) expression in various lysates
PTPN11 / SHP-2 / NS1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PTPN11 / SHP-2 / NS1 Antibody - Staining A431 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PTPN11 / SHP-2 / NS1 Antibody - Western blot analysis of SHP-2 phosphorylation expression in A431 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PTPN11 / SHP-2 / NS1 Antibody - Western blot analysis of Phospho-SHP-2 (Tyr542) expression in various lysates
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Polyclonal Rabbit anti‑Human PTPN11 / SHP‑2 / NS1 Antibody (phospho‑Tyr542, IHC, IF, WB) LS‑C801159

Polyclonal Rabbit anti‑Human PTPN11 / SHP‑2 / NS1 Antibody (phospho‑Tyr542, IHC, IF, WB) LS‑C801159

Antibody:
PTPN11 / SHP-2 / NS1 Rabbit anti-Human Polyclonal (pTyr542) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801159-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PTPN11 / SHP-2 / NS1 Rabbit anti-Human Polyclonal (pTyr542) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NS1 antibody LS-C801159 is an unconjugated rabbit polyclonal antibody to NS1 (PTPN11 / SHP-2) (pTyr542) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PTPN11 / SHP-2 / NS1
Synonyms
PTPN11 | Bptp-3 | BPTP3 | CFC | Csw | Corkscrew csw | Gene corkscrew | Phosphatase corkscrew | PTP-2C | SHP2 | PTP-1D | PTP2C | SHP-2 | SHPTP2 | Noonan syndrome 1 | SH-PTP2 | SH-PTP3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human SHP-2 around the phosphorylation site of Tyrosine 542.
Epitope
pTyr542
Specificity
Phospho-SHP-2 (Tyr542) Antibody detects endogenous levels of SHP-2 only when phosphorylated at Tyrosine 542.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTPN11 / SHP-2 / NS1
Q06124 NM_002834 NP_002825.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/17/2024