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PTPN11 / SHP-2 / NS1 Antibody (aa2-216) LS-C17830

Antibody

PTPN11 / SHP-2 / NS1 Antibody (aa2-216) LS-C17830

Antibody:
Rabbit Polyclonal to Mouse PTPN11 / SHP-2 / NS1
Application:
ICC, WB, IP, Neut
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$710 
LS-C17830-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse PTPN11 / SHP-2 / NS1
Application:
ICC, WB, IP, Neut
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
NS1 antibody LS-C17830 is an unconjugated rabbit polyclonal antibody to NS1 (PTPN11 / SHP-2) from human, mouse and rat. Validated for ICC, IP, Neut and WB.

Target
Mouse PTPN11 / SHP-2 / NS1
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC (10 µg/ml)
  • Western blot (1 - 4 µg/ml)
  • Immunoprecipitation (1 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)
Immunogen
GST fusion protein containing aa2-216 of murine SHP 2/SHPTP-2.
Epitope
aa2-216
Specificity
Recognizes murine SHP 2/SHPTP-2 at 64kD. Apparent size increases to ~70kD upon tyrosine phosphorylation. Species cross-reactivity: Human and rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-4 ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts. Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1 ug/ml for this application. Immunoprecipitation: 6 ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells. Immunocytochemistry: 10 ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells. Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 40% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTPN11 / SHP-2 / NS1
Q06124 NM_002834 NP_002825.3

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Reactivity: Human
Range: 0.156-10 ng/ml
Product
Species: Human
Applications: Western blot, ELISA
Reactivity: Human
Range: 31.2-2000 pg/ml
Reactivity: Human
Range: 0.312-20 ng/ml
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence

Requested From: United States
Date Requested: 7/19/2019
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