NS1 antibody LS-C17830 is an unconjugated rabbit polyclonal antibody to NS1 (PTPN11 / SHP-2) from human, mouse and rat. Validated for ICC, IP, Neut and WB.
Mouse PTPN11 / SHP-2 / NS1
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- ICC (10 µg/ml)
- Western blot (1 - 4 µg/ml)
- Immunoprecipitation (1 µg/ml)
- (applications tested for the base form of this product only)
GST fusion protein containing aa2-216 of murine SHP 2/SHPTP-2.
Recognizes murine SHP 2/SHPTP-2 at 64kD. Apparent size increases to ~70kD upon tyrosine phosphorylation. Species cross-reactivity: Human and rat.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-4 ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts. Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1 ug/ml for this application. Immunoprecipitation: 6 ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells. Immunocytochemistry: 10 ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells. Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 40% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About PTPN11 / SHP-2 / NS1