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| Catalog Number | Size | Price |
|---|---|---|
| LS-C745317-25 | 25 µl (1.15 mg/ml) | $304 |
![PTENP1 Antibody - Western blot using the affinity purified anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35 µg of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ptenp1-antibody-n-terminus-ls-c745317/445761_3396293.jpg)
Polyclonal Rabbit anti‑Human PTENP1 Antibody (N‑Terminus, WB) LS‑C745317
Polyclonal Rabbit anti‑Human PTENP1 Antibody (N‑Terminus, WB) LS‑C745317
Antibody:
PTENP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PTENP1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PTENP1 antibody LS-C745317 is an unconjugated rabbit polyclonal antibody to human PTENP1 (N-Terminus). Validated for ELISA and WB.
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
Epitope
N-Terminus
Specificity
A BLAST analysis was used to suggest cross reactivity with PTEN proteins from mouse, dog sources based on 82% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:160000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Source:
E. coli
Tag:
His
Source:
E. coli
Tag:
His
Species:
Bovine, Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
