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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C745317-25 25 µl (1.15 mg/ml) $235 
PTENP1 Antibody - Western blot using the affinity purified anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6).  The band at ~55 kDa (arrowhead) corresponds to PTEN-P1.  Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.  The identity of lower molecular weight bands in lane 4 is unknown.  Briefly, each lane contains approximately 35 µg of lysate.  Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.  The membrane was washed and reacted with a 1:10,000 dilution of conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results.

PTENP1 Antibody LS‑C745317

PTENP1 Antibody LS‑C745317

Antibody:
Rabbit Polyclonal to Human PTENP1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C745317-25
25 µl
1.15 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PTENP1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PTENP1 antibody LS-C745317 is an unconjugated rabbit polyclonal antibody to human PTENP1. Validated for ELISA and WB.
Target
Human PTENP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:160000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTENP1

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Requested From: United States
Date Requested: 10/19/2019