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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSME1 Antibody (aa72‑121) IHC‑plus™ LS‑B8233
Note: This antibody replaces LS-C112209
PSME1 antibody LS-B8233 is an unconjugated rabbit polyclonal antibody to PSME1 from human, mouse, bat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8233-50
50 µg
$485

Popular PSME1 Products

Immunohistochemical analysis of PA28 alpha staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
PSME1 Antibody IHC of formalin-fixed and paraffin-embedded hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA

Product Description

PSME1 antibody LS-B8233 is an unconjugated rabbit polyclonal antibody to PSME1 from human, mouse, bat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About PSME1
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Q06323 NM_006263 NP_006254.1

PSME1 Antibody, 29-kD MCP activator subunit Antibody, IFI5111 Antibody, IGUP I-5111 Antibody, REG-alpha Antibody, REGalpha Antibody, PA28A Antibody, Interferon-gamma IEF SSP 5111 Antibody, PA28alpha Antibody, Proteasome activator subunit-1 Antibody


Specifications

Target
Human PSME1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Bat, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 7.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PSME1 antibody was raised against synthetic peptide located between aa72-121 of human PSME1 (Q06323, NP_788955). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Elephant, Panda, Bat, Pig (100%); Rat, Dog, Bovine, Rabbit, Guinea pig (92%); Mouse (85%).
Blocking Peptide
LS-E21412 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8233. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa72-121
Specificity
Human PSME1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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