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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSME1 Antibody (aa72‑121, Biotin) LS‑C465143
PSME1 antibody LS-C465143 is a biotin-conjugated rabbit polyclonal antibody to PSME1 from human, mouse, bat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C465143-100
100 µl (1 mg/ml)
$385

Popular PSME1 Products

Immunohistochemical analysis of PA28 alpha staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Bat, Pig
Applications: IHC, IHC - Paraffin, Western blot
PSME1 Antibody IHC of formalin-fixed and paraffin-embedded hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry of paraffin-embedded human testis tissue at dilution 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA

Product Description

PSME1 antibody LS-C465143 is a biotin-conjugated rabbit polyclonal antibody to PSME1 from human, mouse, bat and other species. Validated for IHC and WB.
About PSME1
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Q06323 NM_006263 NP_006254.1

PSME1 Antibody, 29-kD MCP activator subunit Antibody, IFI5111 Antibody, IGUP I-5111 Antibody, REG-alpha Antibody, REGalpha Antibody, PA28A Antibody, Interferon-gamma IEF SSP 5111 Antibody, PA28alpha Antibody, Proteasome activator subunit-1 Antibody


Specifications

Target
Human PSME1
Host
Rabbit
Reactivity
Human, Chimpanzee, Gibbon, Monkey, Mouse, Bat, Pig (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Dog, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PSME1 antibody was raised against synthetic peptide located between aa72-121 of human PSME1 (Q06323, NP_788955). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Elephant, Panda, Bat, Pig (100%); Rat, Dog, Bovine, Rabbit, Guinea pig (92%); Mouse (85%).
Epitope
aa72-121
Specificity
Human PSME1
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PSME1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8233 dilution 5-7.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PSME1 antibody LS-B8233 Western blot of Fetal Lung lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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