Target
Mouse PRSS3 / Trypsin 3
Synonyms
PRSS3 | Brain trypsinogen | Mesotrypsin | PRSS4 | Serine protease 4 | Serine protease 3 | TRY4 | Trypsin III | Trypsin IV | Trypsinogen 4 | MTG | Trypsinogen 5 | Pancreatic trypsinogen III | Protease, serine, 3 | Trypsin-3 | Mesotrypsinogen | T9 | TRY3 | Trypsinogen IV
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
NS0-derived rmTrypsin 3 (aa16-246).
Specificity
Selected for its ability to recognize mouse Trypsin 3 in direct ELISAs and Western Blots.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA
Usage
Suitable for use in Western Blot and Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse Trypsin 3. The detection limit for rmTrypsin 3 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 20% cross-reactivity with rhTrypsin 1, rhTrypsin 2, and rhTrypsin 3. Immunoprecipitation: This antibody has been used to immunoprecipitate rmTrypsin 3 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Trypsin 3. The detection limit for rmTrypsin 3 is approximately 3 ng/well.
Reconstitution
Reconstitute in deionized water
Storage
Store lyophilized powder at 4°C for short term only. Reconstitute in PBS with 40-50% glycerol, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee