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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C18918-100 100 µg (1 mg/ml) $425 
PROS1 / Protein S Antibody - Anti-GSK3A Antibody - Western Blot. Western blot of Affinity Purified anti-GSK3A antibody shows detection of a 52 kD band corresponding to human GSK3A present in ~35 ug of HEK293 whole cell lysate (lane 1). A 4-20% Tris Glycine gel was used for separation followed by blocking with 5% BLOTTO in PBS. Primary antibody was used at a 1:1000 dilution in blocking buffer and reacted overnight at 4C. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal PROS1 / Protein S Antibody (aa1‑25, IF, WB) LS‑C18918

Polyclonal PROS1 / Protein S Antibody (aa1‑25, IF, WB) LS‑C18918

Antibody:
Rabbit Polyclonal to Human PROS1 / Protein S
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18918-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PROS1 / Protein S
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Protein S antibody LS-C18918 is an unconjugated rabbit polyclonal antibody to human Protein S (PROS1 ). Validated for ELISA, IF and WB.
Target
Human PROS1 / Protein S
Synonyms
PROS1 | Protein S (alpha) | Protein S alpha | Protein Sa | PS22 | PS21 | Vitamin K-dependent protein S | THPH5 | THPH6 | PROS | PS24 | Protein S | PS23 | PS25
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near aa 1-25 of human GSK3 alpha.
Epitope
aa1-25
Specificity
This antibody will react with both the phosphorylated and non-phosphorylated form of the protein at the S21residue. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and rat based on 100% homology for the immunogen sequence. Cross reactivity with GSK3A homologues from other sources has not been determined. No cross reactivity is expected against GSK3B.
Usage
This affinity-purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kD in size corresponding to GSK3A by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PROS1 / Protein S
P07225 NM_000313 NP_000304.2

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Requested From: United States
Date Requested: 2/21/2020