Human Proliferation Marker IPO-38

Mouse

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgM,k Monoclonal

Unconjugated

Protein A purified

Unmodified

Spleen cells of a human patient with hairy cell leukemia.

Reacts with a 14-16kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot (1:50)
• Immunoprecipitation
• ELISA (1:1000)

Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry frozen and formalin/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. ELISA: 1:1000. Immunohistochemistry:1:50 to 1:100. Western Blot: 1:50 to 1:100. Positive control: Tonsilar tissue.

PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.