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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C662942-100 100 µg $325 
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody
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Monoclonal PROC / Protein C Antibody (WB) LS‑C662942

Monoclonal PROC / Protein C Antibody (WB) LS‑C662942

Antibody:
Mouse Monoclonal to Human PROC / Protein C
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C662942-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human PROC / Protein C
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Protein C antibody LS-C662942 is an unconjugated mouse monoclonal antibody to human Protein C (PROC ). Validated for ELISA and WB.
Target
Human PROC / Protein C
Synonyms
PROC | Anticoagulant protein C | Blood coagulation factor XIV | Autoprothrombin IIA | Protein C | PROC1 | Vitamin K-dependent protein C | THPH3 | THPH4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
E. coli-derived human Protein C recombinant protein (Position: D200-P461).
Specificity
Plasma; synthesized in the liver.
Presentation
4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3 per 100ug antibody
Reconstitution
Reconstitute with 0.2ml distilled H2O.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PROC / Protein C
P04070 NM_000312 NP_000303.1

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Requested From: United States
Date Requested: 2/25/2020