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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C312837-100 100 µg (0.5 mg/ml) $355 
PROC / Protein C Antibody - PROC / Protein C antibody. IHC(P): Human Breast Cancer Tissue.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Cell Lysate Lane 2: CEM Cell Lysate Lane 3: SMMC Cell Lysate Lane 4: HELA Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - WB of PROC / Protein C antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HELA Cell Lysate.
PROC / Protein C Antibody - Flow Cytometry analysis of A549 cells using anti-PROC antibody. Overlay histogram showing A549 cells stained with anti-PROC antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PROC / Protein C Antibody - PROC / Protein C antibody. IHC(P): Human Breast Cancer Tissue.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Cell Lysate Lane 2: CEM Cell Lysate Lane 3: SMMC Cell Lysate Lane 4: HELA Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - WB of PROC / Protein C antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HELA Cell Lysate.
PROC / Protein C Antibody - Flow Cytometry analysis of A549 cells using anti-PROC antibody. Overlay histogram showing A549 cells stained with anti-PROC antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal PROC / Protein C Antibody (aa446‑461, IHC, WB) LS‑C312837

Polyclonal PROC / Protein C Antibody (aa446‑461, IHC, WB) LS‑C312837

Note: This antibody replaces LS-C389133
Antibody:
Rabbit Polyclonal to Human PROC / Protein C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C312837-100
100 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human PROC / Protein C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Protein C antibody LS-C312837 is an unconjugated rabbit polyclonal antibody to human Protein C (PROC) (aa446-461). Validated for IHC and WB.
Target
Human PROC / Protein C
Synonyms
PROC | Anticoagulant protein C | Blood coagulation factor XIV | Autoprothrombin IIA | Protein C | PROC1 | Vitamin K-dependent protein C | THPH3 | THPH4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Protein C(446-461 aa HGHIRDKEAPQKSWAP).
Epitope
aa446-461
Specificity
Plasma; synthesized in the liver.
Presentation
Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide per 100 ug antibody.
Reconstitution
200 µl Distilled water
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PROC / Protein C
P04070 NM_000312 NP_000303.1

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Requested From: United States
Date Requested: 4/1/2020