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Antibodies > PROC / Protein C > LS-C312837
 
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Catalog Number Size Price
LS-C312837-10 10 µg $318 
LS-C312837-100 100 µg (0.5 mg/ml) $470 
PROC / Protein C Antibody - PROC / Protein C antibody. IHC(P): Human Breast Cancer Tissue.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Cell Lysate Lane 2: CEM Cell Lysate Lane 3: SMMC Cell Lysate Lane 4: HELA Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - WB of PROC / Protein C antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HELA Cell Lysate.
PROC / Protein C Antibody - Flow Cytometry analysis of A549 cells using anti-PROC antibody. Overlay histogram showing A549 cells stained with anti-PROC antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PROC / Protein C Antibody - PROC / Protein C antibody. IHC(P): Human Breast Cancer Tissue.
PROC / Protein C Antibody - Western blot analysis of Protein C using anti-Protein C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Cell Lysate Lane 2: CEM Cell Lysate Lane 3: SMMC Cell Lysate Lane 4: HELA Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.
PROC / Protein C Antibody - WB of PROC / Protein C antibody. Lane 1: JURKAT Cell Lysate. Lane 2: CEM Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HELA Cell Lysate.
PROC / Protein C Antibody - Flow Cytometry analysis of A549 cells using anti-PROC antibody. Overlay histogram showing A549 cells stained with anti-PROC antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human PROC / Protein C Antibody (aa446‑461, IHC, WB) LS‑C312837

Polyclonal Rabbit anti‑Human PROC / Protein C Antibody (aa446‑461, IHC, WB) LS‑C312837

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Note: This antibody replaces LS-C389133
Antibody:
PROC / Protein C Rabbit anti-Human Polyclonal (aa446-461) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312837-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PROC / Protein C Rabbit anti-Human Polyclonal (aa446-461) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Protein C antibody LS-C312837 is an unconjugated rabbit polyclonal antibody to human Protein C (PROC) (aa446-461). Validated for IHC and WB.
Target
Human PROC / Protein C
Synonyms
PROC | Anticoagulant protein C | Blood coagulation factor XIV | Autoprothrombin IIA | Protein C | PROC1 | Vitamin K-dependent protein C | THPH3 | THPH4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Protein C(446-461 aa HGHIRDKEAPQKSWAP).
Epitope
aa446-461
Specificity
Plasma; synthesized in the liver.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PROC / Protein C
P04070 NM_000312 NP_000303.1

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Featured Products

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Species: Human
Applications: ELISA
PROC / Protein C Antibody - Anti-PROC / Protein C antibody IHC of human vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PROC / Protein C Antibody LS‑B4229
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Immunoelectrophoresis
PROC / Protein C Antibody - MCF7 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PROC / Protein C Antibody (aa182‑231) LS‑C80453
Species: Human
Applications: Western blot
PROC / Protein C Antibody - IHC of paraffin-embedded Breast ca, using Protein C antibody at 1:250 dilution.
PROC / Protein C Antibody LS‑C155242
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
PROC / Protein C Antibody (HRP) LS‑C38497
Species: Human
Applications: ELISA
Product
PROC / Protein C Antibody (clone PC107, APC) LS‑C131689
Species: Human
Applications: Western blot, ELISA
See all PROC / Protein C Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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