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Catalog Number Size Price
LS-C742544-100 100 µg (0.5 mg/ml) $503 
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with Goat anti-PRKD1 (aa233-246) Antibody (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with antibody (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtai
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug antibody. The precipitate was subsequently probed in Western blot using antibody at 1ug/ml. The secondary anti-goat picks up the heavy chain of antibody used
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug Goat anti-PRKD1 (aa233-246) Antibody. The precipitate was subsequently probed in Western blot using Goat anti-PRKD1 (aa233-246) Antibody at 1ug/ml. The secondary anti-goat picks up the heavy chain of Goat anti-PRKD1 (aa233-246) Antibody used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with Goat anti-PRKD1 (aa233-246) Antibody (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with antibody (0.1ug/ml) in top panel, probed with anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtai
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug antibody. The precipitate was subsequently probed in Western blot using antibody at 1ug/ml. The secondary anti-goat picks up the heavy chain of antibody used
PRKD1 / PKC Mu Antibody - HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug Goat anti-PRKD1 (aa233-246) Antibody. The precipitate was subsequently probed in Western blot using Goat anti-PRKD1 (aa233-246) Antibody at 1ug/ml. The secondary anti-goat picks up the heavy chain of Goat anti-PRKD1 (aa233-246) Antibody used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA
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Polyclonal Goat anti‑Human PRKD1 / PKC Mu Antibody (aa233‑246, IHC, WB) LS‑C742544

Polyclonal Goat anti‑Human PRKD1 / PKC Mu Antibody (aa233‑246, IHC, WB) LS‑C742544

Antibody:
PRKD1 / PKC Mu Goat anti-Human Polyclonal (aa233-246) Antibody
Application:
IHC, WB, IP, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C742544-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PRKD1 / PKC Mu Goat anti-Human Polyclonal (aa233-246) Antibody
Application:
IHC, WB, IP, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKC Mu antibody LS-C742544 is an unconjugated goat polyclonal antibody to human PKC Mu (PRKD1) (aa233-246). Validated for IHC, IP, Peptide-ELISA and WB.
Target
Human PRKD1 / PKC Mu
Synonyms
PRKD1 | PKCM | Pkcmu | Protein kinase C, mu | Protein kinase D | NPKC-mu | PRKCM | Protein kinase C mu | Protein kinase D1 | NPKC-D1 | PKC-MU | PKD | Protein kinase C mu type
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QKSPSESFIGREKR, from the internal region of the protein sequence according to NP_002733.2.
Epitope
aa233-246
Applications
  • IHC
  • Western blot (0.1 - 0.3 µg/ml)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3 ug/ml IHC: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Dappler et al, Sci Rep. 2016 Sep 21;6:33758 PMID 27649783. Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKD1 / PKC Mu
Q15139 NM_002742 CAA53384.1

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Requested From: United States
Date Requested: 4/18/2024