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Antibodies > PRKCH / PKC-Eta > LS-C407769
 
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Catalog Number Size Price
LS-C407769-10 10 µg $318 
LS-C407769-100 100 µg $470 
PRKCH / PKC-Eta Antibody - PKC eta antibody Western blot. All lanes: Anti PKC eta at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: A549 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: SKOV Whole Cell Lysate at 40 ug. Predicted band size: 78 kD. Observed band size: 78 kD.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody. Overlay histogram showing MCF-7 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody. Overlay histogram showing K562 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody. Overlay histogram showing A431 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PRKCH / PKC-Eta Antibody - PKC eta antibody Western blot. All lanes: Anti PKC eta at 0.5 ug/ml. Lane 1: A431 Whole Cell Lysate at 40 ug. Lane 2: A549 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: SKOV Whole Cell Lysate at 40 ug. Predicted band size: 78 kD. Observed band size: 78 kD.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody. Overlay histogram showing MCF-7 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody. Overlay histogram showing K562 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PRKCH / PKC-Eta Antibody - Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody. Overlay histogram showing A431 cells stained with anti-PKC-eta antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human PRKCH / PKC‑Eta Antibody (aa30‑389, WB) LS‑C407769

Polyclonal Rabbit anti‑Human PRKCH / PKC‑Eta Antibody (aa30‑389, WB) LS‑C407769

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Antibody:
PRKCH / PKC-Eta Rabbit anti-Human Polyclonal (aa30-389) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407769-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKCH / PKC-Eta Rabbit anti-Human Polyclonal (aa30-389) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Eta antibody LS-C407769 is an unconjugated rabbit polyclonal antibody to human PKC-Eta (PRKCH) (aa30-389). Validated for WB.
Target
Human PRKCH / PKC-Eta
Synonyms
PRKCH | PKCL | Protein kinase c eta | Protein kinase C, eta | PKC-L | PRKCL | NPKC-eta | PKC eta | Protein kinase C eta type
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human PKC eta recombinant protein (Position: R30-D389). Human PKC eta shares 96.7% and 96.1% amino acid (aa) sequence identity with mouse and rat PKC eta, respectively.
Epitope
aa30-389
Specificity
Most abundant in lung, less in heart and skin. .
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCH / PKC-Eta
P24723 NM_006255 NP_006246.2

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PRKCH / PKC-Eta Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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